Antibody

β-Amyloid protofibrils, β-Amyloid N-terminus

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Clonality: monoclonal
Host: Mouse
Isotype: IgG2
Source: PFA1
(lab of Chris Dealwis,
Univ. of Tennessee, Knoxville, TN)
Reference:
Gardberg, 2007
Formulation: liquid, protein-A affinity purified
Reactivity:
Reacts in: Human

Immunogen/Epitope

immunogen = CLC-stabilized protofibril form of Aβ(1–40); epitope within Aβ(1–8); DAEFRHDS, at the N terminus

Specificity

The Fab fragments exhibit binding to Aβ monomers in the 20–40 nM range, and this binding is significantly impaired or eliminated in Aβ(1–40) mutants in which a single residue in the 3–7 segment is replaced with alanine. The full IgG molecules bind significantly better than Fab fragments to Aβ fibrils and protofibrils (0.1–0.2 nM); PFA1 binds more tightly to fibrils than does PFA2. diminished affinity for pyroglutamate-Glu forms of Aβ

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References

Paper Citations

  1. Gardberg AS, Dice LT, Ou S, Rich RL, Helmbrecht E, Ko J, Wetzel R, Myszka DG, Patterson PH, Dealwis C. Molecular basis for passive immunotherapy of Alzheimer's disease. Proc Natl Acad Sci U S A. 2007 Oct 2;104(40):15659-64. PubMed.

External Citations

  1. Chris Dealwis

Further Reading

No Available Further Reading