Protocols
This section contains experimental protocols of specific importance to Alzheimer's disease research. As we rely on the research community to help us build and improve this resource, we encourage you to consider contributing your protocols for inclusion. We also welcome suggestions for information you would like to see included in the database, and encourage you to email us at protocols@alzforum.org.
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Breeding & Genotyping
Cell Culture
- Coating of Transwell Filters
- Collagenase/Dispase Mouse Brain Isolation
- Culturing Embryonic Fibroblasts
- MDCK Cell Monolayer and Aβ Transport Assay
- Magnetofection Transfection of Murine Embryonic Motor Neurons
- Primary Astroglial Cultures From Mouse Cerebral Cortex
- Primary Microglial Cultures From Mouse Cerebral Cortex
- Primary Mixed Glial Cultures From Mouse Cerebral Cortex
- Primary Support Cultures of Hippocampal and Substantia Nigra Neurons
Collection/Processing of Biofluids
- Alzheimer's Association Flow Chart for Lumbar Puncture CSF Processing
- Biofluid Sample Collection Protocol for ADNI
- Cerebrospinal Fluid (CSF) Collection and Processing
- Plasma Fluid Collection and Processing
ELISA
Enzyme Activity Assays
Histology
- Acid Phosphatase Histochemistry for Mouse Brain
- Horseradish Peroxidase (HRP) Uptake/Trafficking
- Thioflavin S Staining
Immunostaining
- Immunohistochemistry (Immunoperoxidase)
- Immunohistochemistry for Brain Sections (ABC and Immunofluorescence)
- Immunostaining for Neprilysin
Microscopy
Nucleic Acid Isolation/Purification
Protein Isolation/Extraction
- Extraction of Aβ From Brain for Application to Aβ ELISA
- Extraction of Human Aβ from APP Tg mouse brains
Protein Isolation/Purification
Tissue Preparation
Western Blotting
Other
Members can use our online form to submit a protocol for consideration.
Submit Protocol