Antibody

Tau (Alz50)

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Clonality: monoclonal
Host: Mouse
Isotype: IgM
Source: Formerly available through Dr. Peter Davies. For updated information, see AlzAntibodies page linked in Note below.
Reactivity:
Recognizes a misfolded conformation of tau.

Immunogen/Epitope

Raised against human basal forebrain homogenate. Epitope subsequently determined to include amino acids 2-10 and 312-342 of tau.

Specificity

Recognizes a misfolded conformation of tau. 68 kDa Tau. Stains neuronal components of AD brain specimens but not normal brain specimens.

Note

Please see new entry for Alz-50 in the AlzAntbodies database.

Comments / Questions

  1. This antibody recognizes a folded conformation of tau containing amino acids 2-10 and 312-342. We have used it successfully to detect misfolded tau in mouse models of tauopathy using array tomography, a relatively new imaging technique (Kopeikina et al., 2013).

    References:

    Kopeikina KJ, Polydoro M, Tai HC, Yaeger E, Carlson GA, Pitstick R, Hyman BT, Spires-Jones TL. Synaptic alterations in the rTg4510 mouse model of tauopathy. J Comp Neurol. 2013 Apr 15;521(6):1334-53. PubMed.

    View all comments by Tara Spires-Jones

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References

AlzAntibodies Citations

  1. Tau (Alz-50)

Further Reading

Papers

  1. Shahani N, Subramaniam S, Wolf T, Tackenberg C, Brandt R. Tau aggregation and progressive neuronal degeneration in the absence of changes in spine density and morphology after targeted expression of Alzheimer's disease-relevant tau constructs in organotypic hippocampal slices. J Neurosci. 2006 May 31;26(22):6103-14. PubMed.
  2. Tanemura K, Chui DH, Fukuda T, Murayama M, Park JM, Akagi T, Tatebayashi Y, Miyasaka T, Kimura T, Hashikawa T, Nakano Y, Kudo T, Takeda M, Takashima A. Formation of tau inclusions in knock-in mice with familial Alzheimer disease (FAD) mutation of presenilin 1 (PS1). J Biol Chem. 2006 Feb 24;281(8):5037-41. PubMed.
  3. Tatebayashi Y, Miyasaka T, Chui DH, Akagi T, Mishima K, Iwasaki K, Fujiwara M, Tanemura K, Murayama M, Ishiguro K, Planel E, Sato S, Hashikawa T, Takashima A. Tau filament formation and associative memory deficit in aged mice expressing mutant (R406W) human tau. Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13896-901. PubMed.
  4. Lewis J, Dickson DW, Lin WL, Chisholm L, Corral A, Jones G, Yen SH, Sahara N, Skipper L, Yager D, Eckman C, Hardy J, Hutton M, McGowan E. Enhanced neurofibrillary degeneration in transgenic mice expressing mutant tau and APP. Science. 2001 Aug 24;293(5534):1487-91. PubMed.
  5. Wittmann CW, Wszolek MF, Shulman JM, Salvaterra PM, Lewis J, Hutton M, Feany MB. Tauopathy in Drosophila: neurodegeneration without neurofibrillary tangles. Science. 2001 Jul 27;293(5530):711-4. Epub 2001 Jun 14 PubMed.
  6. Jicha GA, Bowser R, Kazam IG, Davies P. Alz-50 and MC-1, a new monoclonal antibody raised to paired helical filaments, recognize conformational epitopes on recombinant tau. J Neurosci Res. 1997 Apr 15;48(2):128-32. PubMed.
  7. Kosik KS, Orecchio LD, Binder L, Trojanowski JQ, Lee VM, Lee G. Epitopes that span the tau molecule are shared with paired helical filaments. Neuron. 1988 Nov;1(9):817-25. PubMed.
  8. Uéda K, Masliah E, Saitoh T, Bakalis SL, Scoble H, Kosik KS. Alz-50 recognizes a phosphorylated epitope of tau protein. J Neurosci. 1990 Oct;10(10):3295-304. PubMed.