β-Amyloid protofibrils, β-Amyloid N-terminus
PFA1(lab of Chris Dealwis,Univ. of Tennessee, Knoxville, TN)Reference: Gardberg, 2007
monoclonal
IgG2
Mouse
(liquid, protein-A affinity purified)
-
Human
immunogen = CLC-stabilized protofibril form of Aβ(1–40); epitope within Aβ(1–8); DAEFRHDS, at the N terminus
The Fab fragments exhibit bindingto Aβ monomers in the 20–40 nM range, and this binding issignificantly impaired or eliminated in Aβ(1–40) mutants inwhich a single residue in the 3–7 segment is replaced withalanine. The full IgG molecules bind significantly better than Fabfragments to Aβ fibrils and protofibrils (0.1–0.2 nM); PFA1 binds more tightly to fibrils than doesPFA2. diminished affinity forpyroglutamate-Glu forms of Aβ
-
β-Amyloid protofibrils, β-Amyloid N-terminus
PFA2(lab of Chris Dealwis,Univ. of Tennessee, Knoxville, TN)Reference: Gardberg, 2007
monoclonal
IgG2
Mouse
(liquid, protein-A affinity purified)
-
Human
immunogen = CLC-stabilized protofibril form of Aβ(1–40); epitope within Aβ(1–8); DAEFRHDS, at the N terminus
The Fab fragments exhibit bindingto Aβ monomers in the 20–40 nM range, and this binding issignificantly impaired or eliminated in Aβ(1–40) mutants inwhich a single residue in the 3–7 segment is replaced withalanine. The full IgG molecules bind significantly better than Fabfragments to Aβ fibrils and protofibrils (0.1–0.2 nM); PFA1 binds more tightly to fibrils than doesPFA2. diminished affinity forpyroglutamate-Glu forms of Aβ
-
Aβ
Aβ 1-42(Frederique Bard,Elan Pharmaceuticals, So. San Francisco, CA)Reference: Bard, 2003
polyclonal
Unknown Host
(Ig, ammmonium sulfate precip.)
-
-
immunogen = full length amyloid beta 1-42; epitope at aa 1-11
amyloid-beta
-
CREB1 phos Ser133
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(biotinylated)
-
Dog, Human, Mouse, Rat
immunogen = synthetic peptide corresponding to aa 125-135 of CREB phosphorylated at Ser133
CREB phosphorylated at Ser133; also with ATF1 phosphorylated at Ser63
-
CREB1 phos Ser133
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(protein G purified)
-
Human
immunogen = synthetic peptide containing phosphorylated serine at position 133 of human CREB (KLH-coupled)
CREB, phosphorylated (Ser133)
-
ATF-1 [H-60]
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = peptide corresp. to aa 1-60 mapping at the N-terminus of human ATF-1
ATF-1
-
ATF-2 [C-19]
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat, Xenopus
Immunogen = peptide mapping at the C-terminus of ATF-2
ATF-2
-
ATF-3 [C-19]
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide, gelatin)
-
Human, Mouse, Rat
Immunogen = peptide mapping at the C-terminus of human ATF-3
ATF-3
-
CREB binding protein [C-1]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
Immunogen = peptide mapping at the C-terminus of human CBP
CBP p265
-
CREB binding protein [A22]
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = peptide mapping at the N-terminus of human CBP
CBP p265
-
CREB binding protein [C-20]
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = peptide mapping at the C-terminus of human CBP
CBP p265
-
CREB-2 [C-19]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = peptide mapping at the C-terminus of human CREB-2
CREB-2
-
CRE-BPa [C-17]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified; blocking peptide sc-46076P)
-
Human, Mouse, Rat
Immunogen = peptide mapping at the C-terminus of human CRE-BPa
CRE-BPa
-
CRE-BPa [G-16]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified; blocking peptide sc-46078P)
-
Human
Immunogen = peptide mapping at the internal region of human CRE-BPa
CRE-BPa
-
Cdc2
LifeSpan Biosciences, Inc.
monoclonal
Mouse
-
Amphibian (various), Rodent (various), Chicken, Human, Mouse, Xenopus
immunogen =C-terminal fragment starting at aa85 of Xenopus p34cdc2 expressed in E. coli ; epitope is thought to be residues 220-227 of mouse cdc2, L G T P N N E V
cdc2
-
Cyclin B2[C-13]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide)
-
Human
immunogen = peptide mapping at the C-terminus of human cyclin B2
cyclin B2
-
Cyclin B2[K-16]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide)
-
Mammal (various), Human, Mouse, Rat
immunogen = peptide mapping at internal region of mouse cyclin B2
cyclin B2
-
Cyclin B2[N-20]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide)
-
Mammal (various), Human, Mouse, Rat
epitope = mapping at N-terminus of human cyclin B2
cyclin B2
-
Cyclin B[dN-17]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Drosophila
epitope = mapping at N-terminus of drosophila melanogaster cyclin B
cyclin B
-
p21 (M-19)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Mammal (various), Cat, Human, Mouse, Rat
immunogen = epitope mapping at the C-terminus of mouse p21
p21; partially cross-reactive with the related mitotic inhibitory protein, p27
-
p21 (N-20)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified)
-
Human
immunogen = epitope mapping at the N-terminus of mouse p21
p21
-
p19ARF [G-19]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Mouse, Rat
immunogen = epitope mapping at the N-terminus of mouse p19 ARF
detects p19ARF
-
PARP-1 [A-20]
Santa Cruz
polyclonal
IgG
Goat
(affinity purified, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = a peptide mapping at the N-terminus of mouse PARP
PARP (A-20) reacts with 112 kDa PARP-1, and the 29 kDa fragment
-
PARP-1 [H-300]
Santa Cruz
polyclonal
IgG
Rabbit
(liquidG, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = aa 1-300 mapping at the N-terminus of PARP-1 of human origin
reacts with 116/85 kDa PARP-1
-
PARP-1[F-2]
Santa Cruz
monoclonal
IgG2a
Mouse
(affinity purified, PBS, azide)
-
Human
immunogen = recombinant protein corr. to a 764-1014 at the C terminus of human PARP-1
PARP (F-2) reacts with 112 kDa PARP, and the 85 kDa apoptosis-related cleavage fragment
-
PARP-1 [N-20]
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = a peptide mapping at the N-terminus of human PARP
PARP (N-20) reacts with 112 kDa PARP-1, and the 29 kDa fragment
-
PARP-2 [A-18]
Santa Cruz
polyclonal
IgG
Goat
(affinity purified, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = a peptide mapping at the N-terminus of human PARP-2
PARP-2
-
CRF [C-20]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide; affinity purified)
-
Pig, Human, Mouse, Rat
immunogen = peptide mapping at the C-terminus of human corticotropin-releasing factor (CRF)
CRF
-
CRF [S-19]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide; affinity purified)
-
Mouse, Rat
immunogen = peptide mapping at the N-terminus of rat CRF
CRF; no cross reaction with the processed active peptide
-
CRF [N-19]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide; affinity purified)
-
Cow, Human
immunogen = peptide mapping at the N-terminus of human CRF
corticoliberin precursor; no cross reaction with the processed active peptide
-
CRF binding protein [C-19]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human
immunogen = peptide mapping at the C-terminus of human corticotropin-releasing factor binding protein (CRF-BP)
CRF-BP
-
CRF binding protein [C-20]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = peptide mapping at the C-terminus of human corticotropin-releasing factor binding protein (CRF-BP)
CRF-BP
-
CRF binding protein [R-19]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human
immunogen = peptide mapping at the C-terminus of rat corticotropin-releasing factor binding protein (CRF-BP)
CRF-BP
-
CRF-RI/II [H-215]
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
immunogen = peptide corresp. to aa 230-444 mapping at the C-terminus human corticotropin-releasing factor receptor 1 (CRF-RI)
CRF-RI/II
-
CRF-RI/II [C-20]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Cow, Human, Mouse, Rat
immunogen = peptide mapping at the C-terminus human corticotropin-releasing factor receptor 1 (CRF-RI)
CRF-RI/II
-
CRF-RII [C-15]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = peptide mapping at the C-terminus human corticotropin-releasing factor receptor II (CRF-RII)
CRF-RII
-
CRF-RII [N-20]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = peptide mapping at the N-terminus mouse corticotropin-releasing factor receptor II (CRF-RII)
CRF-RII
-
CRF-RII [W-17]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = peptide mapping at within an internal region of human corticotropin-releasing factor receptor II (CRF-RII)
CRF-RII
-
α-Tubulin (B-5-1-2)
Abcam
monoclonal
IgG1
Mouse
(liquid, protein-A purified, PBS, azide)
-
Chicken, Sea Urchin, Cow, Human, Mouse, Rat, Chlamydomonas reinhardtii, African green monkey, Kangaroo rat
immunogen = sarkosyl-resistant filaments from sea urchin (epitope at C-terminal end of the α-tubulin isoform
~50 kDa α-Tubulin
-
α-Tubulin (B-5-1-2)
Novus Biologicals
monoclonal
IgG1
Mouse
(liquid, protein-A purified, PBS, azide)
-
Chicken, Sea Urchin, Cow, Human, Mouse, Rat, Chlamydomonas reinhardtii, African green monkey, Kangaroo rat
immunogen = sarkosyl-resistant filaments from sea urchin (epitope at C-terminal end of the α-tubulin isoform
~50 kDa α-Tubulin
-
βII-Tubulin (6B1)
BioLegend
monoclonal
IgG3
Mouse
(purified)
-
Mammal (various)
immunogen = synthetic peptide C E E E E G E D E A found at the C-terminus of β II tubulin
(50 kDa) Class II β-tubulin
Available from BioLegend
βII-Tubulin (7B9)
BioLegend
monoclonal
IgG3
Mouse
(purified)
-
Mammal (various)
immunogen = synthetic peptide C E E E E G E D E A found at the C-terminus of β II tubulin
(50 kDa) Class II β-tubulin
Available from BioLegend
β-Tubulin
US Biological
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, BSA, azide, glycerol)
-
Various species
immunogen = synthetic peptide derived from aa416-430 of human β-tubulin (epitope at aa 416-430)
recognizes human β-tubulin
-
Tubulin (2Q1105)
US Biological
monoclonal
IgG2a
Rat
(liquid, purified, PBS, azide)
-
Non-specific
immunogen = Yeast tubulin (a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged, represented by Gly-Gly-Tyr in Tyr-Tubulin).
recognizes the alpha subunit of tubulin-specificity binding to so called Tyr-Tubulin, which is produced by post translational modification of tubulin
-
α-Tubulin (TU-01)
Biogenesis AdB Serotec
monoclonal
IgG1
Mouse
(liquid, Ig fract, protein-A, PBS, no preservatives)
-
-
immunogen = crude tubulin preparation (epitope at aa 65-79 of alpha tubulin)
recognizes aa 65-79 of alpha tubulin
-
α-Tubulin (H-300)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Mammal (various), Zebra fish, Xenopus, Drosophila
recombinant protein corresp. to aa 149-448 mapping at the C-terminus of human α-Tubulin
α-Tubulin
-
β-Tubulin (αN-18)
Santa Cruz
polyclonal
IgG
Goat
(affinity purified IgG, PBS, gelatin, azide)
-
Corn (zea mays), Arabidopsis thaliana, Pisum sativum
immunogen = peptide mapping at the N- terminus of Arabidopsis thaliana beta tubulin
beta tubulin
-
δ-Tubulin (C-19)
Santa Cruz
polyclonal
IgG
Goat
(affinity purified, PBS, gelatin, azide)
-
Human, Mouse, Rat
peptide mapping at the C-terminus of delta tubulin of human origin
delta tubulin
-
δ-Tubulin (H-300)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
recombinant protein corresp. to aa 154-450 mapping at the C-terminus of δ-Tubulin of human origin
δ-Tubulin
-
ε-Tubulin (C-15)
Santa Cruz
polyclonal
IgG
Goat
(affinity purified, PBS, gelatin, azide)
-
Human
mapping at the C-terminus of epsilon tubulin of human origin
epsilon tubulin
-
ε-Tubulin (H-280)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
recombinant protein corresp. to aa 196-475 mapping at the C-terminus of ε Tubulin of human origin
ε-Tubulin
-
γ-Tubulin (D10)
Santa Cruz
monoclonal
IgG2b
Mouse
(liquid, PBS, gelatin, azide)
-
Human
aa. 269-451 at the C-terminus of γ tubulin of human origin
specific for gamma tubulin
-
γ-Tubulin (C-11)
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
aa. 269-451 at the C-terminus of γ tubulin of human origin
specific to gamma tubulin
-
γ-Tubulin (aL-18)
Santa Cruz
polyclonal
IgG
Goat
(affinity purified IgG, PBS, gelatin, azide)
-
Corn (zea mays), Arabidopsis thaliana, Nicotiana tabacum
immunogen = peptide mapping at the N-terminus of Arabidopsis thaliana gamma tubulin
gamma tubulin
-
γ-Tubulin (H-183)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat
recombinant protein corresponding to aa 269-451 mapping at the C-terminus of human γ-Tubulin
γ-Tubulin
-
α-Tubulin (TU-01)
Zymed/ Invitrogen
monoclonal
IgG1
Mouse
(diluted ascites fluid, azide)
-
Various species
(epitope at aa 65-79)
α-tubulin, N-terminal structural domain
-
Tubulin, PolyGlut (B3)
Sigma Aldrich
monoclonal
IgM
Mouse
(liquid, IgG, PBS, BSA, azide)
-
Bovine, Rabbit, Chicken, Sea Urchin, Dinoflagellate, Human, Monkey, Mouse, Rat
immunogen = purified sea urchin sperm axonemal proteins (epitope in C-terminal region of α- and β-tubulins, the glutamylated motif at amino acid 445-457 of α-tubulin)
polyglutamylated C-terminal region of α- and β-tubulin
-
Tubulin, polyglut. (B3)
Novus Biologicals
monoclonal
IgM
Mouse
(liquid, affinity purified, PBS, BSA, azide)
-
Rabbit, Chicken, Sea Urchin, Cow, Human, Mouse, Rat, Macaque
immunogen = purified sea urchin --Lytechinus pictus-- sperm axonemal proteins (epitope localized in the C-terminal region of α- and β- tubulins, the glutamylated motif at aa 445-457 of α-tubulin)
recognizes polyglutamylated tubulin motif at aa 445-457
-
Tubulin, polyglut. (B3)
Abcam
monoclonal
IgM
Mouse
(liquid, protein-A purified, PBS, BSA, azide)
-
Rabbit, Chicken, Sea Urchin, Cow, Human, Mouse, Rat, Macaque
immunogen = purified sea urchin --Lytechinus pictus-- sperm axonemal proteins (epitope localized in the C-terminal region of alpha and beta tubulins, the glutamylated motif at aa 445-457 of alpha tubulin)
recognizes polyglutamylated Tubulin motif at amino acid 445-457
-
p53 [C-19]
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Mammal (various), Dog, Human, Mouse, Rat
immunogen = epitope mapping at the C-terminus of human p53
p53
-
p53 [Pab 246]
Santa Cruz
monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
-
Mouse, Rat
immunogen = epitope mapping at aa residues 88-93 of mouse p53
p53; recognizes wildtype but not mutant p53 under non-denaturing conditions
-
p53 phos Ser392
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(protein A purified)
-
Human, Monkey
immunogen = synthetic phosphopeptide corresp. to aa residues at Ser392
p53 phosphorylated at Ser392
-
p53 phos Ser392
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(DEAE-column chromatography)
-
Human, Mouse
immunogen = peptide surrounding the human p53, phosphorylated at Ser392 and coupled to KLH
recognizes only p53 phosphorylated at Ser392 residue; no reaction with non-phosphorylated p53 or unrelated phospho-proteins
-
p53
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(unpurified serum)
-
Human, Mouse, Rat
immunogen = peptide surrounding the human p53, acetylated at Lys373, coupled to KLH
recognizes only p53 acetylated at K373 residue; no reaction with non-acetylated p53 or unrelated proteins
-
p53
LifeSpan Biosciences, Inc.
polyclonal
Rabbit
(unpurified serum)
-
Human, Mouse, Rat
immunogen = synthetic peptide surrounding the human p53, acetylated at Lys320, KLH coupled. Lys320 was acetylated by PCAF
recognizes only p53 acetylated at K373 residue; no reaction with non-acetylated p53 or unrelated proteins; no crossreaction with p63 or p73
-
p53 phos Ser392
LifeSpan Biosciences, Inc.
monoclonal
Mouse
-
Human
immunogen = synthetic peptide corresp. to a portion of p53 phosphorylated at Ser392
p53 when phosphorylated at Ser392
-