Protocols
PI:
Affiliation:
Submitted by: Dennis Selkoe
Posted Date: 24 Feb 2009
This is a protocol for culturing MDCK cells (an epithelial cell line derived from the canine kidney) in a monolayer and assessing Trans-Epithelial Electrical Resistance (TEER) and Aβ transport.
No Available Further Reading
Protocols
PI:
Affiliation:
Submitted by: Dennis Selkoe
Posted Date: 24 Feb 2009
A protocol for culturing rodent fibroblasts.
No Available Further Reading
No Available Comments
Protocols
PI:
Affiliation:
Submitted by: Dennis Selkoe
Posted Date: 24 Feb 2009
A method for isolating endothelial cells from the mouse brain for cell culture.
No Available Further Reading
No Available Comments
Protocols
PI:
Affiliation:
Submitted by: Dennis Selkoe
Posted Date: 24 Feb 2009
A protocol to coat filters with fibronectin and collagen for cell culture.
No Available Further Reading
No Available Comments
Protocols
PI:
Affiliation:
Submitted by: Dennis Selkoe
Posted Date: 23 Feb 2009
This protocol describes how to use Thioflavin S to detect plaques and tangles in brain tissue.
No Available Further Reading
No Available Comments
Protocols
PI:
Affiliation:
Submitted by: Dennis Selkoe
Posted Date: 23 Feb 2009
This protocol describes how to strip the antibodies from a PVDF membrane so that another protein can be assessed on the same blot.
No Available Further Reading
No Available Comments
Protocols
PI:
Affiliation:
Submitted by: Dennis Selkoe
Posted Date: 23 Feb 2009
A short protocol for preparing mouse brain for analysis by preserving one hemisphere in liquid nitrogen and the other in 10% formalin.
No Available Further Reading
No Available Comments
Protocols
PI:
Affiliation:
Submitted by: Dennis Selkoe
Posted Date: 23 Feb 2009
This is a protocol for measuring the enzymatic activity of neprilysin, an Aβ-degrading enzyme.
No Available Further Reading
No Available Comments
Protocols
PI:
Affiliation:
Submitted by: Dennis Selkoe
Posted Date: 23 Feb 2009
A protocol for enriching for a protein of interest by taking advantage of the specific interaction of an antigen with an antibody.
No Available Further Reading
No Available Comments
Protocols
PI:
Affiliation:
Submitted by: Dennis Selkoe
Posted Date: 23 Feb 2009
A method for detecting proteins of interest in brain sections using an immunoperoxidase reaction.
No Available Further Reading
No Available Comments
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