Protocols

Extraction of Human Aβ from APP Tg mouse brains

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Submitted by: Dennis Selkoe
Posted Date: 23 Feb 2009

Brief Description

Extraction of human Aβ from APP transgenic mouse brain into TBS-soluble, guanidine HCI-soluble fractions.

Further Reading

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Protocols

Coupling Antibodies to Protein A/G

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Submitted by: Dennis Selkoe
Posted Date: 23 Feb 2009

Brief Description

This protocol can be used to generate an affinity matrix consisting of an antibody covalently linked to Protein A/G-coated beads.

Further Reading

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Protocols

ACE Activity Assay

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Submitted by: Dennis Selkoe
Posted Date: 23 Feb 2009

Brief Description

A method to measure angiotensin-converting enzyme (ACE) activity.

References

Further Reading

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Protocols

Aβ ELISA

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Submitted by: Dennis Selkoe
Posted Date: 23 Feb 2009

Brief Description

A protocol to detect Aβ in tissue culture supernatant and in homogenized tissue.

Further Reading

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Protocols

Immunoelectron Microscopy of Brain Tissues Embedded in L R White Resin

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Submitted by: Wenlang Lin
Posted Date: 28 Jan 2009

Brief Description

A protocol for tissue processing and post-embedding immunogold labeling.

References

Further Reading

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