Akt, phos Ser473 (11E6)
Alexis Enzo Life Sciences
monoclonal
IgG1
Mouse
(lyophilized supernatant, azide, PEG, sucrose, purified)
-
Dog, Human, Mouse, Rat
Immunogen = synthetic peptide corresp. to C-terminal (PQFpSYSA) of human Akt (protein kinase B; PKB) phosphorylated at Ser473
Ser473-phosphorylated Akt; no cross-reaction with dephosphorylated Akt or unrelated serine-phosphorylated proteins.
-
Akt, dephos Ser473 (11A11)
Alexis Enzo Life Sciences
monoclonal
IgG1
Mouse
(lyophilized, PBS, azide, PEG, sucrose, purified)
-
Dog, Human, Mouse, Rat
Immunogen = synthetic peptide corresp. to C-terminal (QFpSYS A) of human Akt (protein Kinase B; PKB) dephosphorylated at Ser472/473/474
Akt dephosphorylated at Ser473; no cross-reaction with akt phosphorylated at Ser473
-
Akt1 (6D109)
LifeSpan Biosciences, Inc.
monoclonal
IgG1 , κ
Mouse
(lyophilized, purified)
-
Dog, Human, Mouse, Rat
immunogen = synthetic peptide sequence (aa466-480) located at the C-terminus of Akt (KLH coupled)
Akt1
-
Akt1/2 (N-19)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat, Xenopus
Immunogen = peptide mapping at the N-terminus of human Akt1
Akt1 and Akt2
-
Aβ fibrils, APP CTF (WO2)
WO2(Brian O'Nuallain,Graduate School of Medicine, University of Tennessee Medical Center, Knoxville, TN)PaperReference: Villemagne, 2010
monoclonal
IgM
Mouse
-
Human, Mouse
raised against sonicated amyloid fibrils grown from synthesized Aβ1–40 peptides (epitope at Aβ residues 5–8)
detects amyloid-beta fibrils; exhibits Ab binding to fibrils in the presence of a large excess of Aβ(1–40) monomer, suggesting that Ab is directed against conformational epitopes that only exist in the fibril
-
Amyloid β42 (G2-11)
G2-11Konrad BeyreutherCenter for Molecular Biology Heidelberg (ZMBH), University of HeidelbergPaperReference: Villemagne, 2010
monoclonal
Mouse
(liquid, purified, PBS, BSAAlso see Chemicon MABN12)
-
Human, Mouse
immunogen = synthetic peptide corresponding to Aβ42, aa. 35-42 (MVGGVVIA), conjugated with KLH or BSA through a cysteine residue added at the N terminus of the peptides
recognizes Amyloid β42 at the C-terminus
-
Amyloid β40 (G2-10)
G2-10Konrad BeyreutherCenter for Molecular Biology Heidelberg (ZMBH), University of HeidelbergPaperReference: Villemagne, 2010
monoclonal
Mouse
(liquid, purified, PBS, BSAAlso see Chemicon MABN11)
-
Human, Mouse
immunogen = synthetic peptide corresponding to Aβ40, aa. 33-40 (GLMVGGVV), conjugated with KLH or BSA through a cysteine residue added at the N terminus of the peptides
recognizes Amyloid β40 at the C-terminus
-
Aβ (BAN50)
BAN-50(N Suzuki,Takada Chemical Industries)References:Wang S, 2010Fukumoto, 2010Suzuki, 1994
monoclonal
Mouse
-
Human, Mouse
raised against Aβ 1-16 (epitope at aa 1-10)
amyloid-beta
-
Aβ, N-terminal (1E8)
1E8(Paul Cutler,SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Harlow, Essex, UKpaper: Cutler, et al, l997)
monoclonal
Mouse
-
Bovine, Human
immunogen = the derived peptide sequence aa. 13–22, (epitope at aa. 18-22, VFFAE) of beta amyloid
reacts with the N terminal of amyloid-beta; recognizes Aβ[1–40], Aβ[1–42] and Aβ[1–43]
-
Amyloid β40 (G2-10)
Chemicon and Upstate are Millipore
monoclonal
IgG2b ,κ
Mouse
(liquid, purified, buffer, Tris-glycine, BSA, azide)
-
Human, Mouse
immunogen = linear peptide of human Amyloid β40 at the C terminus
recognizes Amyloid β40 at the C-terminus
-
Amyloid β [1-42]
Innogenetics
kit
Mouse
-
Human
Uses 21F12 as a capture antibody and incubation with biotinylated 3D6; 3D6 selectively binds Aß peptides with a free aminogroup at Asp1 (epitope 1–5)
amyloid beta (cerebrospinal fluid)
-
Aβ 42 (G2-13)
Genetics Company, Inc.
monoclonal
IgG1 ,κ
Mouse
(liquid, stabilizing solution, no azide, affinity purified, conj. HRP)
-
Dog, Human, Guinea Pig, Mouse, Rat, Cynomolgus
immunogen = Aβ 1-42 peptide at the C-terminus
C-terminal of Aβ 1-42 peptide; not Aβ 1-38, Aβ 1-39, Aβ 1-40; low cross reactivity to Aβ 43 (10%)and Aβ 44 (13%)
-
β-Amyloid
Cell Signaling
polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
-
Bovine, Human, Rat
immunogen = synthetic peptide corresp. to residues at the N-terminus of human β-Amyloid peptides
detects several isoforms of β-amyloid peptide (Abeta), such as Abeta-40, Abeta-42 etc, regardless of phosphorylation state
-
Bad pSer155 (27AT937)
Novus Biologicals
monoclonal
IgG1
Mouse
(liquid, PBS, azide, affinity purified)
-
Human, Mouse
immunogen = synthetic peptide corresp. to seq. in Ser155 region of mouse Bad and residue Ser155 is phosphorylated
recognizes BAD only when phosphorylated at Ser 155
-
Bad pSer155 (27AT381)
Novus Biologicals
monoclonal
IgG1
Mouse
(liquid, PBS, azide, affinity purified)
-
Human, Mouse
immunogen = synthetic peptide corresp. to seq. in Ser155 region of mouse Bad and residue Ser155 is phosphorylated
recognizes BAD only when phosphorylated at Ser 155
-
Bad pSer155 (27AT381)
Novus Biologicals
monoclonal
IgG1
Mouse
(liquid, azide, affinity purified)
-
Mouse
immunogen = synthetic phosphopeptide corresp. to residues surrounding Ser155 of mouse Bad, (conjugated with KLH)
Bad - Bcl-associated death promoter (27AT381 ) [Ser155]
-
Bad
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = peptide mapping at the N-terminus of human Bad
Bad
-
Bax
BioGenex
polyclonal
IgG
Rabbit
(affinity purified, PBS, BSA, azide)
-
Human
immunogen = synthetic peptide encompassing a unique epitope at the N-terminus of human Bax protein coupled to KLH
detects the α, β and δ isoforms of bax protein
-
Bax (2D2)
Biosource Invitrogen
monoclonal
IgG1
Mouse
(liquid, ascites, PBS, azide, protein-A purified, unconj.)
-
Human, Monkey
immunogen = synthetic peptide corresp. to a sequence at the N-terminal of the human protein
Bax
-
Bax (2D2)
Biosource Invitrogen
monoclonal
IgG1 , κ
Mouse
(liquid, ascites, PBS, BSA, azide, protein-A purified, unconj.)
-
Human, Monkey
immunogen = synthetic peptide corresp. to a sequence at the N-terminal of the human protein
Bax
-
Bax (6A7)
Biosource Invitrogen
monoclonal
IgG1
Mouse
(liquid, ascites, PBS, azide, protein-A purified, unconj.)
-
Human, Rat
immunogen = synthetic peptide corresp. to a sequence at the N-terminal of the human protein
Bax
-
Bax
Chemicon and Upstate are Millipore
polyclonal
IgG1
Rabbit
(liquid, Tris-glycine, azide, protein A purified)
-
Human
immunogen = linear peptide corresp. to human BAX at and around the N-terminus, KLH conj.
Bax
-
Bax
LifeSpan Biosciences, Inc.
monoclonal
IgG1
Mouse
(liquid, PBS, azide, protein A purified)
-
Human, Monkey
immunogen = synthetic peptide corresp. to a seq. at the N-terminal of human Bax
Bax
-
Bax (2D2)
LifeSpan Biosciences, Inc.
monoclonal
IgG1
Mouse
(liquid, BBS, azide, protein G purified)
-
Human
immunogen = peptide corresp. to aa 3-16 at the N- terminus of human Bax, KLH-conjugated
Bax
-
Bax (6A7)
LifeSpan Biosciences, Inc.
monoclonal
IgG1
Mouse
(liquid, BBS, azide, protein G purified)
-
Human, Mouse, Rat
immunogen = peptide corresp. to aa 12-24 at the N- terminus of human Bax, KLH-conjugated
Bax
-
Bax
LifeSpan Biosciences, Inc.
monoclonal
IgG1
Mouse
(liquid, PBS, azide, protein A purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to a sequence at the N-terminal of the protein which is common to human, mouse and rat
Bax
-
Connexin-26 (N-19)
Santa Cruz
polyclonal
Goat
(affinity purified, liquid, PBS, azide, gelatinAlso available as rabbit rabbit, sc-7261-R)
-
Human, Mouse, Rat
immunogen = a peptide mapping at the N-terminus of connexin 26 of human origin
polyclonal for detection of connexin 26, and toa lesser extent, connexin 30
-
Connexin-43 (C-20)
Santa Cruz
polyclonal
IgG
Goat
(liquid, affinity purified IgG, PBS, azide, gelatin.Also available sc-6560 AC, agarose conj.)
-
Dog, Zebra fish, Cow, Human, Mouse, Rat, Xenopus
immunogen = a peptide mapping at the C-terminus of connexin 43 of human origin
recommended for detection of connexin 43.
-
Connexin-43 (H-150)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid IgG, PBS, azide, gelatin)
-
Zebra fish, Human, Mouse, Rat, Xenopus
immunogen = aminoacids 233-382 mapping at the C-terminus of connexin 43 of human origin
recommended for detection of connexin 43.
-
GRK2 (C-15)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, affinity purified IgG, PBS, azide, gelatinAvailable as agarose (sc-562 AC) conjugate for IP)
-
Bovine, Human, Mouse, Rat
immunogen = a peptide mapping at the C-terminus of GRK 2 of human origin
for detection of GRK2
-
GRK3 (C-14)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, affinity purified IgG, PBS, azide, gelatin)
-
Cow, Human, Mouse, Rat
immunogen = a peptide mapping at the C-terminus of GRK 3 of bovine origin
for detection of GRK3
-
GRK6 (C-20)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, affinity purified IgG, PBS, azide, gelatinAvailable as agarose conjugate for IP, sc-566 AC,)
-
Bovine, Human, Mouse, Rat
immunogen = a peptide mapping at the C-terminus of GRK 6 of human origin
for detection of GRK6
-
CK-IIα' (C-20)
Santa Cruz
polyclonal
IgG1
Goat
(liquid, PBS, azide, gelatin, affinity purified Also available as agarose conj. for IP, Cat# sc-6481 AC)
-
Bovine, Human, Mouse, Rat
immunogen = epitope mapping at the C-terminus of human Casein Kinase IIα '
detects Casein Kinase IIα'
-
Tau, truncated at Asp421 (C3)
TauC3(Vincent L. Cryns, Cell Death Regulation Laboratory, Division of Endocrinology,Metabolism, and Molecular Medicine, Northwestern University, Chicago, IL)Research paperReference: Alonso, 2010
monoclonal
IgG1
Mouse
(purified IgG, HEPES, saline, glycerol.)
-
Human
immunogen = KLH- C S S T G S I D M V D, corresp. to residues 412-421 at the C-terminus of tau truncated at Asp421
recognizes tau when truncated at Asp421; no reaction with full-length tau, or with tau truncated at Glu391 or Ala429
-
acetylated Tau /ac-tau
Ab708Li Gan, Gladstone Institute of Neurological DiseaseAssistant Professor of NeurologyUniversity of California, San FranciscoReference: Min SW, 2010
polyclonal
IgG2b
Rabbit
-
Human, Rat
immunogen = a synthetic tau peptide (amino acids 160–182 for 2N4R tau isoform) containing acetylated lysines at positions 163 and 174 and 180
Ab708 specifically recognizes tau acetylated by p300 under cell-free conditions. In HEK293T cells transfected with tau, overexpression of p300 markedly elevatedthe levels of ac-tau detected with Ab708 while the increase in the levels of t-tau was modest, suggesting that Ab708 preferentially recognizes p300-induced ac-tau in cultured cells. Ab708 recognizes human tau acetylated at positions 163, 174, or 180 and possibly other acetylated lysines on tau, but not nonacetylated tau
-
Tau (12E8)
Philip Dolan, Prothena Biosciences, South San Francisco, CA
monoclonal
Mouse
-
Mammal (various), Human, Zebra fish, Drosophila, C. elegans
immunogen = tau phosphorylated at S262 in KXGS motif of the 4th repeat and/or Ser356
tau phosphorylated at S262 and/or S356
-
Tau (DA9)
Formerly available through Dr. Peter Davies. For updated information, contact antibodies@alzforum.org.
monoclonal
IgG1
Mouse
-
Human, Mouse
epitope at aa102–140
recognizes total tau
-
Tau (AT8)
-
monoclonal
IgG1 κ
Mouse
(liquid, PBS, sterile filtered, affinity purified)
-
Human
partially purified human tau, aa. around phosphorylated Serine 202
Recognizes phosphorylated paired helical filament tau. Recognises tau protein phosphorylated at both serine 202 and threonine 205. No cross-reactivity with unphoshorylated tau.
-
Tau, phospho 181P
Innogenetics
kit
Unknown Host
(solid-phase ELISA, uses monoclonal HT7 for capture, AT270 biotinylated for incubation, streptavidin; working range: 25 to 150 pg/mL)
-
Human
specific for tau phosphorylated at threonine 181, antigen in cerebrospinal fluid (CSF)
-
Tau pS199/202
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
tau-derived peptide that is phospho. at serines 199 and 202
tau phosphorylated at serines 199 and 202
-
Tau pS202
Biosource Invitrogen
polyclonal
Rabbit
(affinity purified Ig)
-
Human
immunogen = human tau-derived synthetic peptide that is phosphorylated at serine 202
tau phosphorylated at serine 202
-
Tau pS262
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol)
-
Human
human tau-derived peptide that is phosphorylated at serine 262
tau phosphorylated at serine 262
-
Tau pS409
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at serine 409
tau phosphorylated at serine 409
-
Tau pT212
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at threonine 212
tau, tau phosphor. at threonine 212
-
Tau pT217
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at threonine 217
tau phosphorylated at threonine 217
-
Tau pT231
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at threonine 231
tau phosphorylated at threonine 231
-
Tau pS404
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at serine 404
tau phosphorylated at serine 404
-
Tau pS400
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at serine 400
tau phosphorylated at serine 400
-
Tau, truncated, cleavage site 421/422 (Tau-C3)
Biosource Invitrogen
monoclonal
IgG1 ,κ
Mouse
(affinity purified ascites, PBS, BSA, azide, unconj.)
-
Human, Mouse, Rat
immunogen = KLH- CSSTGSIDMVD, corresp. to the C-terminus of tau truncated at Asp421
recognizes tau when truncated at Asp421; no reaction with full-length tau, or with tau truncated at Glu391 or Ala429
-
Tau pT181
Biosource Invitrogen
kit
Unknown Host
(contains:Hu Tau [pT181]Antibody Bead Concentrate;Hu Tau [pT181]Detection Ab Concentrate;Hu Tau [pT181] Standard)
-
Human
human tau-derived peptide that is phosphorylated at threonine 181
for the measurement of human Tau when phosphorylated at threonine 181 in CSF and tissueculture media
-
Tau-1 (PC1C6)
Chemicon and Upstate are Millipore
monoclonal
IgG2a
Mouse
(purified Ig, phosphate buffered, lyophilized)
-
Bovine, Human, Rat
immunogen = purified denaturated bovine MAPs (epitope at or near a phosphorylated site)
binds to all known electrophoretic species of tau in human, rat and bovine brain (one-dimensional SDS-PAGE); there is some unphosphorylated bias with clone PC1C6 as it seem to recognize only dephosphorylated serine sites at 195, 198, 199, and 202
-
Tau pT205
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at threonine 205
tau phosphorylated at threonine 205
-
Tau pS422
Biosource Invitrogen
polyclonal
Rabbit
(purified Ig, PBS, BSA, azide, glycerol, unconjugated)
-
Human
human tau-derived peptide that is phosphorylated at serine 422
tau phosphorylated at serine 422
-
Tau (AT270)
Pierce Thermo Scientific
monoclonal
IgG1 κ
Mouse
(supernatant, affinity purified, no carrier or preservativeAlso available biotin conj., MN-1050B)
-
Human
immunogen == partially purified human PHF tau, aa. around phosphorylated Thr181
PHF tau, tamgles and neurofilaments; no cross with recomb. unphospho tau; weak cross with normal tau
-
Tau (AT8)
Pierce Thermo Scientific Cat# MN-1020
monoclonal
IgG1 κ
Mouse
(Protein A affinity purified, PBS, no preservative)
-
Baboon, Human, Rabbit, Hamster, Avian (various), Drosophila
immunogen = partially purified human paired helical filament tau.
Recognizes tau protein phosphorylated at both serine 202 (Ser202) and threonine 205 (Thr205). Does not cross-react with unphosphorylated forms of tau.
Confirmed available April 2015.
Tau (AT180)
Pierce Thermo Scientific
monoclonal
IgG1 κ
Mouse
(purified, no carrier or preservative)
-
Human
immunogen = aa. around phosphorylated Thr231
detects Tau doubly phosphorylated at Thr231 and Ser235; weak recognition of phosphorylation at Thr231 only
-